From GenoSeq
Contents |
Real-Time Quantitative PCR by ABI 7900 TaqMan and Roche LightCycler 480
The UCLA GenoSeq Core provides an ABI PRISM 7900 Sequence Detection System (TaqMan) or Roche LightCycler 480 Real-Time PCR System for Real-Time Quantitative PCR. Applications of this technology include gene expression analysis, mutation screening, allelic discrimination (e.g. SNP detection), dissociation curve with Sybr Green, and pathogen detection and quantitation and High Resolution Melting.
The instrument can be reserved, and the researcher must purchase the reagents and perform the experiments. Core personnel can provide advice on planning and setting up your experiment.
Formats
96 well
384 well
Superarray
Microfluidic card
Primer/Probe Design
The Sequencing Core can also design specific primers and probes for your TaqMan assays. The Core uses proven software that designs primers and probes with the correct Tm, and GC-content, further facilitating accurate sequence detection. You can also try to see if any of the assays you want are available on http://lpgws.nci.nih.gov/cgi-bin/PrimerViewer
Prices
TaqMan run - $40.00 (one plate, regardless of number of samples)
Primer Design - $20.00
Set up a Taqman account with GenoSeq Core by either getting a RECHARGE/ P39 or PURCHASE ORDER.
Learn more about Taqman-Real Time PCR.
Please call us at 310-267-2461 to set up a Taqman account, schedule a run, or if you have any questions. Or contact:
- Uma Dandekar, GenoSeq Core Assistant Director
- Email: uma@ucla.edu
- Phone: 310-267-2461
- Fax: 310-794-5446