From GenoSeq

Contents 1 SEQUENCING GUIDE FOR CUSTOMERS 1.1 Purification of template DNA before sequencing 1.2 Thermocycler profiles for sequencing 1.3 Clean product after sequencing

SEQUENCING GUIDE FOR CUSTOMERS

Purification of template DNA before sequencing

• purify plasmids with QIAprep Spin Miniprep Kit

• purify PACs with QIAGEN Plasmid Kit

• purify PCR reactions with Shrimp Alkaline Phosphatase and Exonuclease I

ExoSAP-IT New!	1000 reactions	US78200
Exonuclease I	2500 reactions	E70073Z
Shrimp Alkaline Phosphatase	500 reactions	E70092Y

• or purify PCR product with QIAGEN QIAquick PCR Purification Kit

At this point, either give the Sequencing Core the template for Full Service processing or continue on as a Ready-to-Run sample.

Thermocycler profiles for sequencing

PCR product Cycle Sequencing

Cycle Sequencing profiles for plasmid and primers the core provides

BAC Cycle Sequencing

Clean product after sequencing

Column purification (strongly recommended for capillary instrument).

• Sequencing Core will perform reaction clean-up for a flat $20.00 charge for up to 96 samples per submission.

• Customer can perform reaction clean-up with columns of their choice or purchase columns from the Sequencing Core.