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Contents

Other Services

Cell Line Authentication

The GenePrint10 from Promega can be used to remove any doubts about Cell Line Identity.


Qubit fluorometer

Reagent/Assay Assay range Sample starting concentration range
Qubit dsDNA HS Assay 0.2–100 ng 10 pg/μl–100 ng/μl
Qubit dsDNA BR Assay 2–1,000 ng 100 pg/μl–1 μg/μl


Agilent 2100 Bioanalyzer

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Our instrument is operated by core personnel only. Use the in-lab sign-up sheet to request the assay you would like us to run when you drop-off your samples. The core personnel will run the chip and output a .pdf file containing the results. If you need the raw data files for your run please indicate this fact on the sign-up sheet. It is up to the researcher to submit their samples within the proper concentration range. Charges are per chip. Depending on the assay you are using each chip holds 11-12 samples as listed below. The approximate turn around time is 1-3 days however, please note that large orders may take longer. We require minimum 3ul per sample.

Bioanalyzer Chips and Kits available at the Core

DNA Kits

Kit name Fragment size (bp) Quantitative range Maximum samples/chip
Agilent High Sensitivity DNA Kit 50 - 7000 5.0-500 pg/ul 11
Agilent DNA 7500 Kit 100 - 7500 0.5-50ng/ul 12
Agilent DNA 1000 Kit 25 - 1000 0.1-50ng/ul 12
Price per Chip- $110

RNA Kits

The Agilent RNA kits with the patented RNA Integrity Number (RIN) are the industry standard for RNA quality assessment. Analysis modes for prokaryotic, eukaryotic, and plant RNA are available for the total RNA kits.

Kit name Quantitative range Maximum samples/chip
total RNA mRNA
Agilent RNA 6000 Pico Kit 50-5000pg/ul 250-5000pg/ul 11
Agilent RNA 6000 Nano Kit 25-500ng/ul 25-250ng/ul 12
Price per Chip- $110



Beckman Z2 COULTER COUNTER

The Coulter Counter can be used for counting cells or particles in the 1-120 micron size range. This technique provides accuracy, speed, versatility, and excellent precision; it is the accepted reference method for cell counting.

Core personnel assisted instrument usage per hour- $40



NanoPhotometer™ Pearl

The IMPLEN NanoPhotometer instrument allows the accurate determination of nucleic acids, proteins and peptides in ultra low sample volumes. For dsDNA it has a detection range of 2 ng/ μl to 18,750 ng/μl.

The NanoPhotometer™ Pearl is equipped with pre-programmed Applications for the following:

Nucleic acids- dsDNA, ssDNA, RNA, and Oligonucleotides, Labeling efficiency
Proteins- Protein UV (A280), Protein Dye, Bradford, Lowry, BCA, Biuret
Price per usage (for up to 50 samples) - $5.00



Please call us at 310-267-2461 if you have any questions or wish to use the instuments for any other aplications.

For more information please contact:

Hemani Wijesuriya
Email: HWijesuriya@mednet.ucla.edu
Phone: 310-267-2461
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