Cell Line Authentication
The GenePrint10 from Promega can be used to remove any doubts about Cell Line Identity.
|Reagent/Assay||Assay range||Sample starting concentration range|
|Qubit dsDNA HS Assay||0.2–100 ng||10 pg/μl–100 ng/μl|
|Qubit dsDNA BR Assay||2–1,000 ng||100 pg/μl–1 μg/μl|
Agilent 2100 Bioanalyzer
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Our instrument is operated by core personnel only. Use the in-lab sign-up sheet to request the assay you would like us to run when you drop-off your samples. The core personnel will run the chip and output a .pdf file containing the results. If you need the raw data files for your run please indicate this fact on the sign-up sheet. It is up to the researcher to submit their samples within the proper concentration range. Charges are per chip. Depending on the assay you are using each chip holds 11-12 samples as listed below. The approximate turn around time is 1-3 days however, please note that large orders may take longer. We require minimum 3ul per sample.
Bioanalyzer Chips and Kits available at the Core
If you quantitated your samples using Nanodrop please aim for your sample dilutions around 5ng/ul and if you quantitated your samples using Qubit, please aim for your sample dilutions around 1ng/ul.
The linear dynamic range depends on the library type and fragment distribution. DNA samples with a broad fragment distribution are at the upper end of the linear dynamic range and DNA libraries with a narrow fragment distribution are at the lower end of the linear dynamic range.
|Kit name||Fragment size (bp)||Quantitative range||Maximum samples/chip|
|Agilent High Sensitivity DNA Kit||50 - 7000||5.0-500 pg/ul||11|
|Agilent DNA 7500 Kit||100 - 7500||0.5-50ng/ul||12|
|Agilent DNA 1000 Kit||25 - 1000||0.1-50ng/ul||12|
Price per Chip- $110
The Agilent RNA kits with the patented RNA Integrity Number (RIN) are the industry standard for RNA quality assessment. Analysis modes for prokaryotic, eukaryotic, and plant RNA are available for the total RNA kits.
|Kit name||Quantitative range||Maximum samples/chip|
|Agilent RNA 6000 Pico Kit||50-5000pg/ul||250-5000pg/ul||11|
|Agilent RNA 6000 Nano Kit||25-500ng/ul||25-250ng/ul||12|
Price per Chip- $110
Beckman Z2 COULTER COUNTER
The Coulter Counter can be used for counting cells or particles in the 1-120 micron size range. This technique provides accuracy, speed, versatility, and excellent precision; it is the accepted reference method for cell counting.
Core personnel assisted instrument usage per hour- $40
The IMPLEN NanoPhotometer instrument allows the accurate determination of nucleic acids, proteins and peptides in ultra low sample volumes. For dsDNA it has a detection range of 2 ng/ μl to 18,750 ng/μl.
The NanoPhotometer™ Pearl is equipped with pre-programmed Applications for the following:
- Nucleic acids- dsDNA, ssDNA, RNA, and Oligonucleotides, Labeling efficiency
- Proteins- Protein UV (A280), Protein Dye, Bradford, Lowry, BCA, Biuret
Price per usage (for up to 50 samples) - $5.00
Please call us at 310-267-2461 if you have any questions or wish to use the instuments for any other aplications.
For more information please contact:
- Hemani Wijesuriya
- Email: HWijesuriya@mednet.ucla.edu
- Phone: 310-267-2461