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DNA Methylation

The GenoSeq Core is offering multiple methods to assess methylation patterns.

High Resolution Melting using Roche Lightcycler 480 : Percent Methylation of a region

Pyrosequencing for small regions using Pyromark MD : upto 50 bases

Pyroseqencing using Roche 454 GS FLX : upto 400 bases


The GenoSeq Core is offering a novel method for assessing DNA methylation using high resolution melting on the Roche LightCycler 480 Real-Time PCR System.

GenoSeq Core Assistant Director Uma Dandekar gave a talk at a Real-Time PCR Workshop, sponsored by Roche Applied Science, at the American Society of Human Genetics Annual meeting in San Diego:

Real-Time PCR Workshop
The LightCycler 480 Real-Time PCR System: Cutting Edge Applications of Real-Time PCR in Human Genetics Studies


Ask about how this can be used in your research when you visit the GenoSeq Core.

For more information please contact:

Hemani Wijesuriya
Email: HWijesuriya@mednet.ucla.edu
Phone: 310-267-2461
Uma Dandekar, GenoSeq Core Assistant Director
Email: uma@ucla.edu
Phone: 310-267-2461
Fax: 310-794-5446

View a video presentation by the GenoSeq Core's Lulu Zhang, on Assessing DNA Methylation


Lulu Zhang and her AACR Poster
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