GS Junior Plus Sequencer
To find out more about using this technology in your research contact us.
Sanger-like read lengths - the power of next-gen throughput. The complete sequencing workflow of the GS Junior Plus Systems comprise of four main steps, leading from purified DNA to analyzed results. These basic steps include:
- Generation of a single-stranded template DNA library:
- Emulsion-based clonal amplification of the library:
- Data generation via sequencing-by-synthesis:
- Data analysis using different bioinformatics tools:
To find out more information about the technology and how the system works please click on the following link The Technology:
|GS Junior Plus System|
|Reads per Run||~100,000 Shotgun 70,000 Amplicon|
|Sample Input||gDNA, cDNA, or amplicons (PCR products)|
|Run Time||18 hours|
Under optimal conditions, ~630-910 million bases of an average read length of ~700 bases and can be obtained with FLXPLUS Chemistry and about ~400-600 million bases of an average read length of ~400 bases and can be obtained with Titanium Chemistry.
- De Novo Sequencing: Whole Genomes of Humans, Plants, Yeasts, Fungi, Bacteria, Viruses, BACS etc.
- Resequencing: SNPs, InDels, Somatic Mutations
- Transcriptome Analysis: cDNA, small non coding RNA, microRNA
- Epigenetic Changes: DNA Methylation Patterns
- Metagenomics: Analysis of Environmental DNA
- Paleogenomics : Ancient DNA
2-4µg of double stranded DNA in more than 10µl volume is required.Ideally DNA size should be larger than 2kb. If your DNA is 500bp long or less, then about 3µg in more than 10µl volume is necessary. The DNA should be suspended in H2O or 1X TE.
Provide the DNA at a concentration > 300ng/µl and A260/280 ratio of approximately 1.8.
An image of DNA sample(s) run on a 1-2% agarose gel is requested to assess quality of incoming DNA. We will further assess the quality using Bioanalyzer and Nanodrop.
De novo sequencing requires 20X average genome coverage. Resequencing and mapping projects require 15X average coverage.
- Amplicon Sequencing
- De Novo Bacteria Sequencing
- Paired End Reads
- Cloning Small RNAs for Sequencing
- RNA Virus Sequencing
High Throughput Sequencing Services Contact Info
- The Sequencing and Genotyping Core is located at
- 36-125 CHS
- UCLA Center for Health Sciences Building
- UCLA Campus Map
- Open 9AM to 5PM Mon-Fri, excluding university holidays
- For more information about sequencing at the Core, call 310-267-2461 or contact:
- Uma Dandekar, GenoSeq Core Assistant Director
- Email: email@example.com
- Phone: 310-267-2461
- Fax: 310-794-5446